Composite

Part:BBa_K3286270

Designed by: Marijn Ceelen   Group: iGEM19_Wageningen_UR   (2019-10-21)


Lambda gpD - GFP fusion

This part allows you to create a fusion protein that combines the capsid decorator protein from phage lambda to GFP. This can theoretically be used to create fluorescent phage lambda. The fusion protein is under the control of Anderson promoter J23116. This is a relatively weak Anderson promoter at 0.16 strength. This choice was made to avoid overexpression of the caspsid fusion, which might make the phage unviable. It also contains a linker consisting of glycines and serines. This should help folding of the protein and increase phage capsid stability.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1045


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